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Electrochemical behavior of the psychotropic drug pivazepam in determinations in pharmaceutical formulations and in urine
Author(s) -
Rivera Paloma,
Bermejo Esperanza,
Zapardiel Antonio,
López José Antonio Pérez,
Hernández Lucas
Publication year - 1991
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140030425
Subject(s) - urine , polarography , detection limit , chromatography , chemistry , urine sample , electrochemistry , differential pulse voltammetry , relative standard deviation , peak current , adsorption , diffusion , standard addition , methanol , pharmaceutical formulation , analytical chemistry (journal) , cyclic voltammetry , inorganic chemistry , electrode , biochemistry , physics , organic chemistry , thermodynamics
The electrochemical reduction of pivazepam was investigated by differential pulse polarography. Reduction of the drug was carried out in one irreversible, diffusion‐controlled step using Britton–Robinson buffers. The peak potential at pH 5.0 and 10% methanol–water solutions was −0.95 V (vs. Ag/AgCl/3M KCl). Pivazepam can be determined (down to the nanomolar level) by using adsorptive preconcentration prior to the differential pulse voltammetric scan. The applicability to assays of commercial preparations and human urine is described. The detection limit was 15 ng per milliliter of urine with 60 seconds adsorption, and the mean standard deviation was lower than 4.6% for 200 ng/mL sample ( n = 5, 60 seconds), with a mean recovery of 82%.