Premium
Peroxidase–ferrocene modified carbon paste electrode as an amperometric sensor for the hydrogen peroxide assay
Author(s) -
Sánchez Pilar Domínguez,
Ordieres Arturo José Miranda,
García Agustín Costa,
Blanco Paulino Tuñón
Publication year - 1991
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140030407
Subject(s) - hydrogen peroxide , amperometry , nafion , ferrocene , chemistry , electrode , horseradish peroxidase , carbon paste electrode , calibration curve , reference electrode , detection limit , analytical chemistry (journal) , chromatography , cyclic voltammetry , inorganic chemistry , electrochemistry , organic chemistry , enzyme
A new enzymatic amperometric sensor for hydrogen peroxide based on a modified carbon paste electrode is described. Horseradish peroxidase and ferrocene mediator were immobilized by dissolution or dispersion in a graphite–paraffin oil paste. To prevent losses of immobilized chemicals under operational conditions, the electrode surface was coated with a Nafion membrane. The device exhibits a relatively fast response time (42 s for 95% of the steady‐state response) and yields currents linearly related to the hydrogen peroxide concentration over the 10 −7 to 10 −5 mol/L range in both static and flow injection operations. The slope of the calibration plot remained constant for more than 20 days. Flow injection assays of hydrogen peroxide at a sampling rate of about 70 injections per hour, with a relative standard deviation of 1.4%, are possible. An easy and low cost preparation procedure is described.