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Flow injection analysis of myoglobin and hemoglobin at toluidine blue chemically modified electrode
Author(s) -
Zhou Jianxun,
Wang Erkang
Publication year - 1991
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140030311
Subject(s) - myoglobin , saturated calomel electrode , toluidine , chemistry , hemeprotein , hemoglobin , electrode , glassy carbon , flow injection analysis , titration , analytical chemistry (journal) , inorganic chemistry , chromatography , cyclic voltammetry , reference electrode , detection limit , electrochemistry , organic chemistry , heme , enzyme
Electrodeposition of the phenothiazine mediator titrant toluidine blue onto a glassy carbon substrate at an appropriate potential was used to construct a toluidine blue chemically modified electrode (CME) exhibiting electrocatalytic reduction for myoglobin and hemoglobin. The CME catalyzed the hemoprotein electroreduction at the reduction potential of the mediator molecule. When the CME as used as a detector for flow injection analysis at a constant applied potential of −0.30 V vs. a saturated calomel electrode, it gave detection limits of 20 and 50 ng (1.2 and 0.78 pmol) injected myoglobin and hemoglobin, respectively, with a dynamic linear concentration range over 2 orders of magnitude. After a brief equilibration period, the CME retained nearly 90% of its initial myoglobin response over 8 hours of continuous exposure to the flow‐through system.