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Sodium activity measurements in protein solutions with sodium amalgam electrodes
Author(s) -
Gunaratna P. C.,
Marinenko G.,
Koch W. F.
Publication year - 1991
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140030209
Subject(s) - aqueous solution , sodium , electromotive force , chemistry , electrode , bovine serum albumin , amalgam (chemistry) , analytical chemistry (journal) , chromatography , organic chemistry , physics , quantum mechanics
A method using sodium amalgam electrodes has been developed to study the effect of protein on activity measurements and residual liquid junction potential of sodium in protein‐containing solutions. It was tested for reliability by application to aqueous sodium chloride solutions before it was applied to protein‐containing solutions. The experimentally determined activity coefficients are in good agreement with the activity coefficients for aqueous sodium chloride solutions reported in literature. Therefore, we used this method to measure the activity of NaCl solutions containing varying concentrations of bovine serum albumin (BSA). The electrode slope of the electrode responses in a protein‐containing medium does not differ from that of pure aqueous solutions. A functional relationship has been established between the concentration of protein and the percent bias in the measurements. Measurements of electromotive force made in protein‐containing solutions do not show any evidence of protein effect on liquid junction potential.