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A polishable amperometric biosensor for bilirubin
Author(s) -
Wang Joseph,
Ozsoz Mebmet
Publication year - 1990
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140020813
Subject(s) - amperometry , bilirubin oxidase , detection limit , bilirubin , biosensor , reproducibility , electrode , horseradish peroxidase , chemistry , chromatography , matrix (chemical analysis) , analyte , analytical chemistry (journal) , electrochemistry , biochemistry , medicine , enzyme , gastroenterology
An amperometric enzyme electrode for bilirubin based on the incorporation of bilirubin oxidase and horseradish peroxidase within a graphite‐epoxy matrix is reported. The resulting bienzyme electrodes are renewable (by polishing) and offer low‐potential detection of bilirubin. The limit of detection is 4 × 10 −6 M and the response is linear up to 1 × 10 −4 M. The influence of various experimental variables is described. The surface‐to‐surface reproducibility is 14% (in terms of relative standard deviation). The fabrication scheme is simple and the response times are short. The prospects of using amperometric bilirubin electrodes for diagnostic work are discussed.