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Amperometric amino acid electrodes
Author(s) -
Pfeiffer Dorothea,
Wollenberger Ulla,
Makower Alexander,
Scheller Frieder,
Risinger Lars,
Johansson Gillis
Publication year - 1990
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140020704
Subject(s) - amperometry , chemistry , amino acid , oxidase test , gelatin , tyrosinase , electrode , lysine , biosensor , chromatography , substrate (aquarium) , d amino acid oxidase , matrix (chemical analysis) , linear range , enzyme , biochemistry , detection limit , electrochemistry , biology , ecology
Substrate specificity, the linear measurement range, and the lifetime of monoenzyme electrodes were determined using L‐glutamate oxidase, L‐lysine oxidase, and tyrosinase immobilized in gelatin. These sensors were applied to the analysis of protein hydrolyzates. By coimmobilizing the three enzymes, an electrode responding to a group of amino acids was obtained. Its analytical performance was compared with that of an L‐amino acid oxidase sensor. The net charge of the immobilization matrix influenced the relative sensitivities of the amino acids.