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Pineapple‐tissue based bioelectrode for the determination of hydrogen peroxide
Author(s) -
Lin Meng Shan,
Tham Sock Ying,
Rechnitz Garry A.
Publication year - 1990
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140020703
Subject(s) - hydrogen peroxide , detection limit , chemistry , flow injection analysis , electrode , peroxidase , relative standard deviation , carbon fibers , nuclear chemistry , inorganic chemistry , chromatography , materials science , biochemistry , enzyme , composite number , composite material
A flow‐injection analysis (FIA) system for the determination of hydrogen peroxide was developed by utilizing a pineapple‐tissue‐based modified carbon paste electrode. Pineapple stem core tissue contains peroxidase, which is not available commercially in the pure isolated form. Hydrogen peroxide concentrations were determined in the presence of O ‐phenylenediamine as a hydrogen donor at a potential of −200 mV. The detection limit (S/N = 3) for hydrogen peroxide using this tissue electrode is 2.1 μM with a relative standard deviation of 0.44% ( n = 12). The tissue‐modified carbon paste electrode is stable for up to 20 days with no significant activity changes.