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Cathodic stripping analysis of DNA/RNA bases in nonaqueous media
Author(s) -
Fish Judith R.,
Ciszewski Aleksander,
Malinski Tadeusz
Publication year - 1990
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140020406
Subject(s) - chemistry , uracil , anodic stripping voltammetry , nucleic acid , tetrabutylammonium hydroxide , cathodic stripping voltammetry , thymine , guanine , dimethyl sulfoxide , inorganic chemistry , voltammetry , hanging mercury drop electrode , supporting electrolyte , deprotonation , hydroxide , ammonium hydroxide , electrochemistry , electrode , ion , dna , organic chemistry , nucleotide , biochemistry , gene
The methods for the determination of nucleic acid bases by differential pulse cathodic stripping voltammetry in dimethyl sulfoxide are proposed and developed. One method uses mercury drop or film electrodes. The required anion is produced by the deprotonation of the parent molecule by added tetrabutyl‐ammonium hydroxide (TBAOH). Optimization of the method is discussed. The effects of deposition potential or time, scan rate, pulse height, TBAOH concentration, and other experimental parameters are examined. Included are the results of the determinations of thymine, uracil, cytosine, adenine, and guanine. The second method uses a mercury‐film rotating ring‐disk electrode. The anion, produced electrochemically at the disk held at a negative potential, is swept to the ring and deposited at a suitable, more positive potential. Detection limis for nucleic acid bases are 10 −8 to 10 −9 M.