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Simultaneous enzymatic determination of glucose and ascorbic acid using flow‐injection amperometry
Author(s) -
Matuszewski Wojciech,
Trojanowicz Marek,
Ilcheva Liliana
Publication year - 1990
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140020211
Subject(s) - ascorbic acid , chemistry , amperometry , glucose oxidase , chromatography , flow injection analysis , resolution (logic) , volumetric flow rate , flow (mathematics) , biochemistry , enzyme , detection limit , electrode , mathematics , electrochemistry , food science , thermodynamics , computer science , physics , artificial intelligence , geometry
A flow‐injection procedure involving the glucose oxidase‐catalyzed oxidation of glucose and direct am‐perometric determination of ascorbic acid was developed for simultaneous determination of the two compounds. Experimental conditions of measurement, including the sample volume, flow rate, and delay coil length, were optimized to obtain satisfactory resolution for the two flow‐injection peaks. The principle of analytical interpretation of the obtained signal is presented, and the applicability of the determination of glucose and ascorbic acid in fresh fruit juices samples is illustrated.