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Electrochemical analysis of oxygen content in samples of whole human saliva
Author(s) -
Cohen Florence,
Burdairon Gerald,
Rouelle François,
Chemla Marius
Publication year - 1989
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140010608
Subject(s) - saliva , oxygen , chemistry , polarography , glucose oxidase , electrochemistry , catalase , clark electrode , quantitative analysis (chemistry) , chromatography , biochemistry , enzyme , electrode , inorganic chemistry , organic chemistry , electrolyte
Although dissolved oxygen plays an important role in chemical and biochemical processes in oral medium, no quantitative result exists concerning its concentration in saliva. Using a micro‐computer operated polarographic device, we could measure the two consecutive waves of O 2 reduction in whole human saliva samples. After correction of the charging current contribution, we have found that the oxygen concentration is quite low, most often less than 0.08 p.p.m. In order to explain this unexpected result, a study of oxygen consumption by enzymatic reactions in saliva has been undertaken. We could demonstrate that enzymatic reactions of glucose oxidase and catalase are responsible for the complete consumption of oxygen in whole saliva samples.