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LCEC determination of the copper protein ceruloplasmin in human serum at a polyaniline chemically modified electrode
Author(s) -
Ye Jiang,
Baldwin Richard P.,
Schlager Janis W.
Publication year - 1989
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140010208
Subject(s) - ceruloplasmin , polyaniline , detection limit , chromatography , amperometry , chemistry , copper , electrode , size exclusion chromatography , polymer , biochemistry , electrochemistry , enzyme , organic chemistry , polymerization
A chemically modified electrode (CME) for use in the amperometric detection of the copper protein ceruloplasmin was constructed by electropolymerizing a polyaniline coating onto glassy carbon. The resultant CME, used in liquid chromatographic detection, gave an electrocatalytic response for ceruloplasmin reduction that could be employed directly by itself or could be greatly enhanced by the addition of Fe 2+ to the mobile phase. Optimum detection was achieved at an applied potential of −0.10 V vs. Ag/AgCl and with 1 mM Fe 2+ in the mobile phase. The detection limit obtained under these conditions was 2 pmol injected, and the response was linear for concentrations at least 2 orders of magnitude higher. The CME retained more than 90% of its initial response to ceruloplasmin over 30 hours of continuous exposure to the chromatographic flowstream. In practice, the liquid chromatography CME response was sensitive and selective enough to permit quantitation of physiologically relevant levels of ceruloplasmin in human serum with no sample pretreatment other than preinjection filtration.