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Amperometric determination of free fatty acids by utilizing five sequential enzyme reactions
Author(s) -
Sode Koji,
Tamiya Eiichi,
Karube Isao,
Kameda Yuko
Publication year - 1989
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140010111
Subject(s) - chemistry , amperometry , enzyme , fatty acid , chromatography , oxygen , electrode , clark electrode , hydrolysis , biochemistry , organic chemistry , electrochemistry , electrolyte
Abstract An enyme electrode for determination of free fatty acids was constructed. Five sequential enzyme reactions are involved in this biosensor. Acyl‐CoA synthetase (EC 6.2.1.3) was used as a specific enzyme for free fatty acids, and produced AMP. AMP was then hydrolyzed with four other sequential enzyme reactions involving oxygen consumption. The oxygen consumed was measured by an oxygen electrode. Using this sensor, a good linearity was obtained between current decrease and free fatty acid concentration below 15 mM. The minimum concentration for the determination was 1.77 mM. The assay procedure was completed within 5 min.