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New approach for the treatment of neuropathic pain: Fibroblast growth factor 1 gene‐transfected adipose‐derived mesenchymal stem cells
Author(s) -
Forouzanfar F.,
Amin B.,
Ghorbani A.,
Ghazavi H.,
Ghasemi F.,
Sadri K.,
Mehri S.,
Sadeghnia H.R.,
Hosseinzadeh H.
Publication year - 2018
Publication title -
european journal of pain
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.305
H-Index - 109
eISSN - 1532-2149
pISSN - 1090-3801
DOI - 10.1002/ejp.1119
Subject(s) - mesenchymal stem cell , medicine , neuropathic pain , adipose tissue , fibroblast growth factor , sciatic nerve , stem cell , pathology , systemic administration , pharmacology , endocrinology , biology , microbiology and biotechnology , receptor , in vivo
Background Neuropathic pain triggered by peripheral nerve lesion is extremely difficult to manage with current approaches, hence the importance of exploring therapeutic alternatives. Methods We have analysed adipose‐derived mesenchymal stem cells ( AD ‐ MSC s) and fibroblast growth factor 1 gene‐transfected adipose‐derived mesenchymal stem cells ( AD ‐ MSC s FGF 1 ) on chronic constriction injury ( CCI ). The mechanical and thermal hypersensitivity were assessed using the von Frey filament, radiant heat and acetone drop tests. Histopathological and apoptotic changes and the level of FGF 1, GFAP and TNF α proteins were assessed in the lumbar portion (L4–L6). Moreover, AD ‐ MSC s FGF 1 were labelled with 99m Tc ‐ HMPAO and isolated organ counting were performed upon AD ‐ MSC s FGF 1 administration. Results Administration of AD ‐ MSC s FGF 1 attenuated the CCI ‐induced mechanical and thermal hypersensitivity. Spinal structural alterations and apoptosis were decreased in the AD ‐ MSC s FGF 1 group. The injection of either phosphate‐buffered saline or normal NIH 3T3 fibroblasts could not attenuate the behavioural symptoms of neuropathic pain. Increased genetically engineered cells were counted in the injured sciatic nerve and the elevated levels of FGF 1 were detected in the spinal tissue. Stem cell therapy lead to decrement the level of the CCI ‐induced TNF ‐α and GFAP expression. Conclusion The intravenous administration of AD ‐ MSC s FGF 1 could be considered as a potential remedy for the management of neuropathic pain. Significance AD ‐ MSC s FGF 1 attenuated the CCI ‐induced mechanical and thermal hypersensitivity. Spinal structural alterations and apoptosis were significantly decreased in the AD ‐ MSC s FGF 1 group. Elevated levels of FGF 1 were detected in the spinal tissue.