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Detergent‐Like Polymerizable Monomers: Synthesis, Physicochemical, and Biochemical Characterization
Author(s) -
Bonnet Christophe,
Guillet Pierre,
Mahler Florian,
Igonet Sébastien,
Keller Sandro,
Jawhari Anass,
Durand Grégory
Publication year - 2020
Publication title -
european journal of organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 1434-193X
DOI - 10.1002/ejoc.202000540
Subject(s) - chemistry , isothermal titration calorimetry , monomer , dynamic light scattering , alkyl , moiety , micelle , vesicle , polymer chemistry , aqueous solution , membrane , organic chemistry , polymer , chemical engineering , biochemistry , nanoparticle , engineering
Three monomers with a maltose polar head, an alkyl hydrogenated chain, and an acrylamide‐based polymerizable moiety were synthesized. The self‐assembly properties in aqueous solutions of these monomers were studied by means of isothermal titration calorimetry (ITC), surface tension (SFT) measurements, and dynamic light scattering (DLS), which indicated the formation of small micellar aggregates of about 6 nm diameter. The critical micellar concentration (CMC) was found to depend on the length of the alkyl chain and on the nature of the polymerizable moiety, ranging from 0.35 m m to ca. 10 m m . The monomers were found to solubilize phospholipid vesicles and to extract a broad range of proteins from Escherichia coli membranes. Finally, the extraction of two membrane proteins, namely, the full‐length, wild‐type human G‐protein‐coupled receptor (GPCR) adenosine A2A receptor (A 2A R) and the bacterial transporter AcrB was demonstrated.