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Reversible Reaction‐Based Fluorescent Probes for Dynamic Sensing and Bioimaging
Author(s) -
Liu Hui,
Wang Sisi,
Gao Hu,
Shen Zhen
Publication year - 2020
Publication title -
european journal of organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 1434-193X
DOI - 10.1002/ejoc.202000359
Subject(s) - chemistry , nucleophile , fluorescence , redox , chemical reaction , covalent bond , chromophore , condensation reaction , nucleophilic addition , reaction dynamics , combinatorial chemistry , molecule , photochemistry , nanotechnology , organic chemistry , catalysis , physics , materials science , quantum mechanics
Diverse chemical species such as ions and molecules exist within living cells and organisms undergoing dynamic changes in their local environment by a web of continuously interacting reactions. Reaction‐based fluorescent probes with a highly sought reversible feature can provide a real‐time monitor of the concentration dynamics (increases and decreases) of such chemical species, thus ideally suited to understand the physiological function, and pathogenic mechanisms of corresponding bio‐species in the regulation of cellular function and disease progression. This review summarizes the current methods for constructing reversible reaction‐based fluorescent probes. The sensing mechanisms and biological applications of these probes are also discussed. The representative examples reported recently are categorized according to the type of reversible chemical reactions utilized: nucleophilic additions (Michael additions, chromophore reactions), nucleophilic addition‐condensation reactions, and redox reactions. Finally, we present the potential challenges and suggestions for developing probes based on dynamic and reversible covalent bond formation reactions.