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Hydroxysteroid Dehydrogenases: An Ongoing Story
Author(s) -
Ferrandi Erica Elisa,
Bertuletti Susanna,
Monti Daniela,
Riva Sergio
Publication year - 2020
Publication title -
european journal of organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 1434-193X
DOI - 10.1002/ejoc.202000192
Subject(s) - chemistry , hydroxysteroid dehydrogenases , cofactor , nad+ kinase , enzyme , steroid , stereochemistry , superfamily , stereoselectivity , biochemistry , hydroxysteroid dehydrogenase , hydroxysteroid , dehydrogenase , catalysis , gene , hormone
Hydroxysteroid dehydrogenases (HSDHs) are NAD(P)(H)‐dependent enzymes which belong to the so‐called short‐chain dehydrogenases/reductases (SDR) superfamily. These oxidoreductases catalyze the oxidation/reduction of hydroxyl/keto groups of steroids with high regio‐ and stereoselectivity. HSDHs were the first group of biocatalysts that was investigated by our group, as an original way to support the ongoing research activity in the area of steroid chemistry in the early seventies. Since then, the research activity on these enzymes has followed three main pathways: a) the isolation and production of new HSDHs; b) the development of new efficient enzymatic systems for the in situ regeneration of the cofactor NAD(P)(H); c) the synthetic exploitation of HSDHs. This minireview presents the results that have been published by various research groups in this area in the last 50 years, with a focus on the selective modification of bile acids.