Premium
Enzymatic Synthesis of 2‐Deoxyribose 1‐Phosphate and Ribose 1 Phosphate and Subsequent Preparation of Nucleosides
Author(s) -
Kulikova Irina V.,
Drenichev Mikhail S.,
Solyev Pavel N.,
Alexeev Cyril S.,
Mikhailov Sergey N.
Publication year - 2019
Publication title -
european journal of organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 1434-193X
DOI - 10.1002/ejoc.201901454
Subject(s) - phosphorolysis , chemistry , ribose , purine nucleoside phosphorylase , deoxyribose , nucleoside , enzyme , phosphate , nucleotide , deoxyguanosine , stereochemistry , thymidine phosphorylase , biochemistry , organic chemistry , nucleic acid , purine , dna , gene
α‐Ribose‐1‐phosphate (Rib‐p) and 2‐deoxy‐α‐ribose‐1‐phosphate (dRib‐p) are key intermediates in nucleoside metabolism and are important starting compounds for the enzymatic synthesis of various modified nucleosides. To date, chemical and enzymatic methods allowed the preparation of these compounds in rather low yields (11–37 %). This prevents their widespread use for the enzymatic synthesis of biologically active and practically important nucleosides. Here we propose to use 7‐methyl‐2′‐deoxyguanosine (7‐Me‐dGuo) and 7‐methylguanosine (7‐Me‐Guo) for the preparation of dRib‐p and Rib‐p. In this paper, we present the effective preparation of Rib‐p and dRib‐p starting from readily prepared 7‐methylguanosine derivatives via their irreversible enzymatic phosphorolysis in the presence of purine nucleoside phosphorylase. Rib‐p and dRib‐P are obtained in nearly quantitative yields (HPLC analysis) and 74–96 % yields after their isolation and purification, which is much higher than previously reported.