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Lipid A Structure and Immunoinhibitory Effect of the Marine Bacterium Cobetia pacifica KMM 3879 T
Author(s) -
Di Lorenzo Flaviana,
Palmigiano Angelo,
AlbitarNehme Sami,
Pallach Mateusz,
Kokoulin Maxim,
Komandrova Nadezhda,
Romanenko Lyudmila,
Bernardini Maria Lina,
Garozzo Domenico,
Molinaro Antonio,
Silipo Alba
Publication year - 2018
Publication title -
european journal of organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 1434-193X
DOI - 10.1002/ejoc.201800279
Subject(s) - lipid a , chemistry , bacteria , escherichia coli , secretion , mass spectrometry , acylation , marine bacteriophage , lipopolysaccharide , microbiology and biotechnology , biochemistry , stereochemistry , biology , gene , immunology , chromatography , genetics , catalysis
The structural elucidation of lipopolysaccharides (LPSs) from Gram‐negative marine bacteria, along with the assessment of their immunological properties, is a fascinating and active research field. Such studies can aid understanding of adaptation phenomena that occur in the marine environment, but they can also open up new perspectives on the design and development of new immunoregulatory drugs. In this paper, we report the structural characterization of the lipid A component of the LPS isolated from the marine bacterium Cobetia pacifica KMM 3879 T , which is characterized by a family of structures differing in their acylation patterns. The structural assignment was achieved through extensive chemical analysis and matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry. Moreover, cellular immunology studies on the LPS highlighted its low immunostimulatory impact, as well as a very interesting and promising inhibitory activity of the toxic effects of Escherichia coli LPS.