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Optimising Terpene Synthesis with Flow Biocatalysis
Author(s) -
Tang Xiaoping,
Allemann Rudolf K.,
Wirth Thomas
Publication year - 2017
Publication title -
european journal of organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 1434-193X
DOI - 10.1002/ejoc.201601388
Subject(s) - biocatalysis , chemistry , sesquiterpene , flow chemistry , terpene , biochemical engineering , farnesyl diphosphate synthase , organic chemistry , enzyme , catalysis , atp synthase , reaction mechanism , engineering
Sesquiterpenes are an important family of natural products, many of which exhibit important pharmaceutical and agricultural properties. They are biosynthesised from farnesyl diphosphate in sesquiterpene synthase catalysed reactions. Here, we report the development of a highly efficient segmented flow system for the enzyme‐catalysed continuous flow production of sesquiterpenes. Design of experiment (DoE) methods were used to optimise the performance of the flow biocatalysis, and quantitative yields were achieved by using an operationally simple but highly effective segmented flow system.