Premium
In Vitro Photodynamic Studies of a BODIPY‐Based Photosensitizer
Author(s) -
Kim Bosung,
Sui Binglin,
Yue Xiling,
Tang Simon,
Tichy Michael G.,
Belfield Kevin D.
Publication year - 2017
Publication title -
european journal of organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 1434-193X
DOI - 10.1002/ejoc.201601054
Subject(s) - singlet oxygen , photosensitizer , chemistry , photodynamic therapy , bodipy , propidium iodide , photochemistry , quantum yield , fluorescence , programmed cell death , apoptosis , oxygen , organic chemistry , biochemistry , physics , quantum mechanics
A new halogenated 4,4‐difluoro‐4‐bora‐3a,4a‐diaza‐ s ‐indacene (BODIPY) derivative was designed as a singlet‐oxygen photosensitizer (PS) for use as a photodynamic therapy (PDT) agent, and its photophysical properties were characterized. The PS, having two iodine atom substituents, exhibited a low fluorescence quantum yield ( Φ FL ) of 0.02 and high singlet‐oxygen‐generation quantum yield ( Φ Δ ) of 0.93. Furthermore, in vitro photodynamic activities were evaluated to assess the potential of using the PS for PDT. A propidium iodide (PI) fluorescence assay and live‐cell imaging of Lewis lung carcinoma (LLC) cells demonstrated that the cellular damage is induced by the photosensitization of the BODIPY dye, which subsequently leads to cell death by necrosis.q