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A BODIPY‐Based Water‐Soluble Fluorescent Probe for Mitochondria Targeting
Author(s) -
Sui Binglin,
Tang Simon,
Woodward Adam W.,
Kim Bosung,
Belfield Kevin D.
Publication year - 2016
Publication title -
european journal of organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 1434-193X
DOI - 10.1002/ejoc.201600238
Subject(s) - bodipy , fluorophore , chemistry , fluorescence , colocalization , biocompatibility , mitochondrion , biophysics , quantum yield , photochemistry , solubility , fluorescence microscope , cytotoxicity , biochemistry , organic chemistry , in vitro , microbiology and biotechnology , physics , quantum mechanics , biology
A water‐soluble fluorescent mitochondrial targeting probe is reported. The probe bears a BODIPY‐based fluorophore, two triphenylphosphonium (TPP) groups as mitochondrial targeting moieties, and two PEG groups to increase water solubility and enhance biocompatibility. As a mitochondria‐selective fluorescent probe, the new BODIPY derivative exhibited many desirable properties, such as water solubility, high fluorescence quantum yield, solvent independent photophysical properties, pH insensitivity, good photostability, low cytotoxicity, and excellent mitochondrial targeting ability. Fluorescence microscopy colocalization experiments demonstrated the ability of this new probe to target mitochondria within living cells for bioimaging.