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Functionalization of the meso ‐Phenyl Ring of Rhodamine Dyes Through S N Ar with Sulfur Nucleophiles: Synthesis, Biophysical Characterizations, and Comprehensive NMR Analysis (Eur. J. Org. Chem. 2/2015)
Author(s) -
Mitronova Gyuzel Yu.,
Polyakova Svetlana,
Wurm Christian A.,
Kolmakov Kirill,
Wolfram Thomas,
Meineke Dirk N. H.,
Belov Vladimir N.,
John Michael,
Hell Stefan W.
Publication year - 2015
Publication title -
european journal of organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 1434-193X
DOI - 10.1002/ejoc.201590000
Subject(s) - chemistry , sted microscopy , fluorophore , fluorescence , rhodamine , ring (chemistry) , molecule , xanthene , photochemistry , stimulated emission , organic chemistry , optics , laser , physics
The cover picture shows the interplay of diffusing light at dusk over the see. In contrast, the doughnut‐shaped laser light used for stimulated emission depletion (STED) fluorescence nanoscopy is well spatially localized; it may be represented as a doughnut with a zero intensity point in the very center. The fluorescence of the molecules exposed to STED light (“covered” by the doughnut) is efficiently inhibited, and the area in which the molecules can fluoresce may be “squeezed” to a spot with dimensions that are no longer limited by diffraction. The structures, synthesis and properties of the rather photostable fluorophores are discussed in the article by G. Yu. Mitronova, V. N. Belov, M. John, S. W. Hell et al. on 337 ff . An additional functional group in the meso ‐phenyl ring attached to the xanthene fluorophore is a key feature of all rhodamine dyes discussed in this report. NMR studies and calculations established the positions of the substituents in the polysubstituted phenyl ring. The authors are grateful to Mr. H. Sebesse (MPI BPC) for his help in creating the cover picture.