Premium
Towards the Sequence‐Selective Recognition of Double‐Stranded DNA Containing Pyrimidine‐Purine Interruptions by Triplex‐Forming Oligonucleotides
Author(s) -
Hari Yoshiyuki,
Obika Satoshi,
Imanishi Takeshi
Publication year - 2012
Publication title -
european journal of organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 1434-193X
DOI - 10.1002/ejoc.201101821
Subject(s) - oligonucleotide , chemistry , pyrimidine , nucleic acid , nucleobase , dna , purine , base pair , combinatorial chemistry , sequence (biology) , triple helix , computational biology , stereochemistry , biochemistry , biology , enzyme
Triplex formation with double‐stranded DNA (dsDNA) by oligonucleotides has potential for applications in attractive technologies such as gene therapy and genetic diagnosis. However, triplex‐forming oligonucleotides (TFOs) can only recognize homopurine strands in homopurine‐homopyrimidine regions in dsDNA, either through Hoogsteen or through reverse‐Hoogsteen hydrogen bonds. A straightforward and powerful approach to overcoming this sequence limitation is the development of artificial nucleic acids capable of recognizing specific pyrimidine‐purine interruptions (i.e., a CG or TA base pair) in triplex formation. This review describes artificial nucleic acids, especially those containing non‐natural nucleobases, developed to recognize CG or TA base pairs in dsDNA targets.