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Structural Analysis of the Capsular Polysaccharide from Acinetobacter lwoffii F78
Author(s) -
Hanuszkiewicz Anna,
Kaczyński Zbigniew,
Lindner Buko,
Goldmann Torsten,
Vollmer Ekkehard,
Debarry Jennifer,
Heine Holger,
Holst Otto
Publication year - 2008
Publication title -
european journal of organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 1434-193X
DOI - 10.1002/ejoc.200800887
Subject(s) - chemistry , polysaccharide , acetylation , nuclear magnetic resonance spectroscopy , mass spectrometry , stereochemistry , acinetobacter , bacteria , biochemistry , chromatography , genetics , biology , gene , antibiotics
The capsular polysaccharide from Acinetobacter lwoffii F78 was isolated and purified, and its structure was elucidated by chemical analyses, NMR spectroscopy, and mass spectrometry. The presence of a capsule on this bacterium was confirmed by transmission electron microscopy experiments, and the utilization of the antilipid A monoclonal antibody proved the nonendotoxin origin of the isolated material. The structure represents a novel nonbranched aminopolysaccharide with high heterogeneity of amino groups substituents: →3)‐α‐ L ‐FucNAc‐(1→3)‐β‐ D ‐QuiNR 1 4NR 2 ‐(1→4)‐β‐ L ‐GlcNR 3 3NR 4 A‐(1→, where R 1 = 3‐hydroxybutyric acid (3‐HBA) or N ‐acetylated alanine (AlaNAc), R 2 = AlaNAc or 3‐HBA, R 3 = acetyl or 3‐HBA, and R 4 = 3‐HBA or acetyl.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)