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The Outer Membrane of the Marine Gram‐Negative Bacterium Alteromonas addita is Composed of a Very Short‐Chain Lipopolysaccharide with a High Negative Charge Density
Author(s) -
Leone Serena,
Molinaro Antonio,
Sturiale Luisa,
Garozzo Domenico,
Nazarenko Evgeny L.,
Gorshkova Raisa P.,
Ivanova Elena P.,
Shevchenko Liudmila S.,
Lanzetta Rosa,
Parrilli Michelangelo
Publication year - 2007
Publication title -
european journal of organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 1434-193X
DOI - 10.1002/ejoc.200600906
Subject(s) - chemistry , lipopolysaccharide , alteromonas , lipid a , glucosamine , heptose , residue (chemistry) , oligosaccharide , bacterial outer membrane , stereochemistry , moiety , bacteria , cell envelope , biochemistry , escherichia coli , medicine , biology , mutant , gene , genetics , endocrinology
The complete structure of the lipopolysaccharide isolated from the Gram‐negative marine bacterium Alteromonas addita , type strain KMM 3600 T = R10SW13 T , has been elucidated by means of a combined chemical approach and state‐of‐the‐art NMR and MS analyses. Isolation and characterisation of the lipid A moiety and the core oligosaccharide were pursued separately after either acid or alkaline treatment of the lipopolysaccharide. The structure detected was identified as a novel, highly negatively charged, deep‐rough lipopolysaccharide in which a trisaccharide subunit is connected to a typical lipid A glucosamine backbone. Within the core oligosaccharide, a phosphodiester bridge connects a glucose unit to a heptose residue.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)