Premium
Cover Picture: Divergent and Linear Solid‐Phase Synthesis of PNA Containing Thiazole Orange as Artificial Base (Eur. J. Org. Chem. 15/2005)
Author(s) -
Jarikote Dilip V.,
Köhler Olaf,
Socher Elke,
Seitz Oliver
Publication year - 2005
Publication title -
european journal of organic chemistry
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 1434-193X
DOI - 10.1002/ejoc.200590030
Subject(s) - peptide nucleic acid , chemistry , dna , thiazole , combinatorial chemistry , nucleobase , base pair , stereochemistry , orange (colour) , biochemistry , food science
The cover picture shows the divergent synthesis of peptide nucleic acid based probes in which the DNA intercalator dye thiazole orange serves as a base surrogate. The so‐called FIT ( F orced I ntercalation of T hiazole orange) PNA probes can provide information about the hybridization to complementary DNA by enhancement of fluorescence. The graphical representation depicted in the bottom left corner gives an idea of the structure of FIT PNA complexed by DNA. In this model, FIT PNA has been hybridized to DNA that contains an abasic site opposite to thiazole orange. The article by O. Seitz et al. on p. 3187 ff. describes the details of linear and divergent solid‐phase syntheses of FIT PNAs. Both methods provide very rapid access and should be applicable to the synthesis of any type of base‐modified PNA molecule.