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A Fluorous Capping Strategy for Fmoc‐Based Automated and Manual Solid‐Phase Peptide Synthesis
Author(s) -
Montanari Vittorio,
Kumar Krishna
Publication year - 2006
Publication title -
european journal of organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 1434-193X
DOI - 10.1002/ejoc.200500958
Subject(s) - chemistry , peptide , peptide synthesis , aqueous solution , combinatorial chemistry , centrifugation , chromatography , phase (matter) , solid phase synthesis , water soluble , aqueous two phase system , organic chemistry , biochemistry
Just add water: Peptides synthesized by the use of standardized Fmoc protocols with commercial automated synthesizers can be purified from deletion products by simple centrifugation of aqueous solutions. The deletion products are capped with fluorous trivalent iodonium salts. At the end of the synthesis, the crude peptide is dissolved in water and centrifuged, and the deletion products precipitate leaving only the full length peptide in solution. Protocols for generalized use of this strategy are reported. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2006)