Solution‐Phase Synthesis of Phosphorothioate Oligonucleotides Using a Solid‐Supported Acyl Chloride with  H ‐Phosphonate Chemistry | Zendy

Adamo Ilaria | Zendy; Dueymes Cécile | Zendy; Schönberger Andreas | Zendy; Navarro AudeEmmanuelle | Zendy; Meyer Albert | Zendy; Lange Meinolf | Zendy; Imbach JeanLouis | Zendy; Link Fritz | Zendy; Morvan François | Zendy; Vasseur JeanJacques | Zendy

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Solution‐Phase Synthesis of Phosphorothioate Oligonucleotides Using a Solid‐Supported Acyl Chloride with H ‐Phosphonate Chemistry

Author(s) -

Adamo Ilaria,

Dueymes Cécile,

Schönberger Andreas,

Navarro AudeEmmanuelle,

Meyer Albert,

Lange Meinolf,

Imbach JeanLouis,

Link Fritz,

Morvan François,

Vasseur JeanJacques

Publication year - 2006

Publication title -

european journal of organic chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.825

H-Index - 155

eISSN - 1099-0690

pISSN - 1434-193X

DOI - 10.1002/ejoc.200500547

Subject(s) - chemistry , random hexamer , phosphonate , oligonucleotide , aqueous solution , solid phase synthesis , chloride , precipitation , oligonucleotide synthesis , solid phase extraction , extraction (chemistry) , combinatorial chemistry , organic chemistry , chromatography , crystallography , dna , biochemistry , peptide , physics , meteorology

The synthesis of oligonucleotides from dimers to a hexamer by a H ‐phosphonate approach in solution using solid‐supported acyl chloride is reported herein. This strategy avoids the use of chromatography. The work‐ups have been simplified and involve filtration, aqueous extraction and precipitation. The synthesis was scaled up to 1 mmol for the dimers and 340 μmol for the hexamer. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2006)

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