Premium
Conformational Study of GPI Anchors: the Common Oligosaccharide GPI Anchor Backbone
Author(s) -
Chevalier Franck,
LopezPrados Javier,
Perez Serge,
MartínLomas Manuel,
Nieto Pedro M.
Publication year - 2005
Publication title -
european journal of organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 1434-193X
DOI - 10.1002/ejoc.200500171
Subject(s) - chemistry , glycosidic bond , intramolecular force , oligosaccharide , cleavage (geology) , molecule , hydrogen bond , stereochemistry , nuclear magnetic resonance spectroscopy , molecular dynamics , computational chemistry , biochemistry , organic chemistry , fracture (geology) , enzyme , geotechnical engineering , engineering
The solution three‐dimensional structure of pseudopentasaccharide 6 , which constitutes the product from the GPI‐PLD cleavage of a GPI anchor, has been studied using a protocol which involves a systematic search of the conformational space around the glycosidic linkages, a thorough molecular dynamics study with explicit water molecules and a full NMR analysis study of intramolecular hydrogen bonding in solution. The results indicate that 6 exists in an extended conformation with a considerable flexibility compatible with a hinge‐like conformational motion. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2005)