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Characterisation of the α‐(1→3) Homopolymer of L ‐ glycero ‐ D ‐ manno ‐Heptose Units Isolated from the O‐Chain Polysaccharide of Agrobacterium radiobacter
Author(s) -
De Castro Cristina,
Sturiale Luisa,
Parrilli Michelangelo
Publication year - 2004
Publication title -
european journal of organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 1434-193X
DOI - 10.1002/ejoc.200400002
Subject(s) - chemistry , heptose , stereochemistry , residue (chemistry) , polysaccharide , bacteria , chemical structure , monosaccharide , biochemistry , organic chemistry , gene , mutant , genetics , biology
Abstract This paper provides the first report of the molecular primary structure of the O‐chain produced from A. radiobacter group M2−1. The repeating unit was determined, by means of chemical and spectroscopical analyses, to have the following structure: [3)‐α‐ L ‐ glycero ‐ D ‐ manno ‐heptose‐(1→] n . The relevant feature of this structure is represented by the particular residue involved: L ‐ glycero ‐ D ‐ manno ‐heptose. This monosaccharide is almost ubiquitous in Gram‐negative bacteria, but it is exclusively located in the Core region and only in one other case has it is reported as an O‐chain constituent. The knowledge of the chemical structure of the O‐chain of A . radiobacter will be useful for comprehending the bacterium‐host recognition mechanism at the molecular level. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2004)