Premium
Metal‐Assisted Hybridization of Oligonucleotides, Evaluation of Circular 2′‐ O ‐Me RNA as Ligands for the TAR RNA Target
Author(s) -
Zapata Laurence,
Bathany Katell,
Schmitter JeanMarie,
Moreau Serge
Publication year - 2003
Publication title -
european journal of organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 1434-193X
DOI - 10.1002/ejoc.200390143
Subject(s) - chemistry , oligomer , oligonucleotide , rna , terpyridine , ligand (biochemistry) , tar (computing) , metal , metal ions in aqueous solution , stereochemistry , crystallography , polymer chemistry , combinatorial chemistry , organic chemistry , dna , biochemistry , receptor , computer science , gene , programming language
Two complementary oligonucleotides were conjugated with terpyridine ligands at their nearby 5′‐ and 3′‐ends. Addition of a stoichiometric amount of a transition metal (Zn 2+ , Fe 2+ ) resulted in a large increase in the melting temperature of the duplex. The conjugation of TPY to stem‐loop oligomers provided an efficient procedure for the cyclisation of the oligomer after the addition of metal ions. Such a short stem‐loop oligomer was designed to target the HIV‐1 TAR RNA through loop−loop interactions. The addition of Zn 2+ ions yielded a good ligand ( K d = 30 nM) for this RNA structural element. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2003)