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Cover Picture: Eur. J. Lipid Sci. Technol. 6∕2018
Publication year - 2018
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.201870040
Subject(s) - phosphatidylcholine , chemistry , phospholipase , docking (animal) , threonine , computer science , enzyme , stereochemistry , biochemistry , phospholipid , medicine , nursing , serine , membrane
Facile Enzymatic Synthesis of Phosphatidylthreonine Using an Engineered Phospholipase D Jasmina Damnjanović, Nozomi Matsunaga, Masaatsu Adachi, Hideo Nakano, and Yugo Iwasaki DOI: 10.1002/ejlt.201800089 One‐step synthesis of phosphatidylthreonine (PtdThr) is established, using the head group exchange of phosphatidylcholine (PtdCho) with Threonine (Thr) catalyzed by an engineered phospholipase D (PLD). The enzyme accepts l ‐Thr, l ‐ allo ‐Thr and d ‐ allo ‐Thr to give the corresponding PtdThr, but not d ‐Thr. Docking simulation explains the enzyme's preference; l ‐, l ‐ allo ‐ and d ‐ allo ‐Thr bind to the active site in a productive orientation, whereas d ‐Thr in a non‐productive orientation.