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1 H LF‐NMR Energy Relaxation Time Characterization of the Chemical and Morphological Structure of PUFA‐Rich Linseed Oil During Oxidation With and Without Antioxidants
Author(s) -
Resende Maysa T.,
Linder Charles,
Wiesman Zeev
Publication year - 2019
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.201800339
Subject(s) - chemistry , polyunsaturated fatty acid , autoxidation , lipid oxidation , antioxidant , linseed oil , chemical structure , degree of unsaturation , lipid peroxidation , organic chemistry , fatty acid
A major stability issue of food and other products containing lipids is their susceptibility to oxidation and the efficacy of endogenous or exogenous antioxidants that are sensitive to internal morphological and chemical structures which are difficult to characterize with current analytical methods. This work develops signal reconstruction of 1 H LF‐NMR spin‐lattice (T 1 ) and spin‐spin (T 2 ) energy relaxation times into 2D T 2 versus T 1 peak graphs of the internal chemical and morphological arrangements within lipid materials, with the potential for efficient structural studies of lipid oxidation and antioxidant efficacy in complex foods. This novel NMR inverse Laplace transformation (ILT) signal processing reconstruction is now applied in a PUFA‐rich linseed oil's thermal autoxidation and antioxidant efficacy study. To characterize antioxidant efficacy, it is important to understand the mechanism of oxidation and the chemical and morphological structural aspects. The initial steps of oxidation show a clear peak shift to shorter T 2 versus T 1 peak values, for the different poly‐unsaturated fatty acids (PUFA), which for mono‐unsaturated and saturated FAs remain unchanged. These peak shifts are within an oxidation time period needed for hydroperoxides formation. For longer oxidation times a new set of T 2 versus T 1 peaks are generated with shorter T 2 and stable and/or increased T 1 values, suggesting formation of aldehydes and polymerized oxidation end products. The chemical changes of the fatty acids of linseed oil are confirmed by their gas chromatography analysis during the studied time interval. Similarly peroxides and aldehydes formation trends are confirmed by PV and p ‐ansidine tests, respectively for the same time points. Based on the oxidation study the control of T 1 and T 2 relaxation times by α tocopherol antioxidant is characterized. In effect α tocopherol antioxidant maintains peak stability during linseed oil's thermal autoxidation, in a 2D T 2 versus T 1 relaxation time graph of peaks assigned to chemical morphological structures, demonstrating α tocopherol's ability to minimize linseed oil's oxidation. Practical Application : Monitoring of food products oxidation and quality control. 1 H LF‐NMR T 1 and T 2 energy relaxation times reconstructed with PDCO into 2D spectra of material analysis has proven to be an advantageous tool to monitor complex chemical and morphological structural changes during the autoxidation of PUFA‐rich linseed oil. It is suggested that an initial oxidation phase generates a shift, of the original linseed peaks along the T 1 = T 2 diagonal due to hydroperoxide formation. At longer oxidation times there is an accumulation of peaks below the diagonal line assigned to oligomeric/polymeric termination products, generating a significant “bending effect.” α tocopherol addition at effective concentrations is able to change this pattern of PUFA‐rich linseed oil oxidation by blocking early peaks shift and late bending effect.