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Isolation, Purification of DPAn‐3 from the Seal Oil Ethyl Ester
Author(s) -
Zheng Zhenxiao,
Dai Zhiyuan,
Cao Yalun
Publication year - 2018
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.201800225
Subject(s) - docosapentaenoic acid , docosahexaenoic acid , eicosapentaenoic acid , chemistry , ethyl ester , yield (engineering) , urea , distillation , chromatography , vacuum distillation , fatty acid , organic chemistry , polyunsaturated fatty acid , materials science , metallurgy
DPA(docosapentaenoic acid)n‐3 is an important ingredient in seal oil and has many potential functions. Yet, it has not been studied systematically for the pure form is difficult to obtain. In this paper, DPAn‐3 with purity of ≈99% and total yield of 44.80% is obtained through the following process. First, eicosapentaenoic acid (EPA), DPAn‐3, and docosahexaenoic acid (DHA) are enriched by urea adduction and the content of them increased from 22.05 to 62.52%. Then, DPA and DPA are concentrated by molecular distillation with a content of them increase from 37.08 to 75.59%. Next, DPAn‐3 is enriched through AgNO 3 complexation and a product with 37.92% of DPA is obtained under the optimal conditions. Finally, DPAn‐3 in the product is purified by preparative HPLC. The process proposed in this paper is specific for the purification of DPA and this work provides important reference for the future studies of DPAn‐3. Practical Applications : DPAn‐3 is a rare fatty acid distributed in marine mammals. Recent studies have proved that it has many potential biological effects on anti‐inflammatory, neuro‐protection, and endothelial cell migration. However, the health benefits of DPAn‐3 have not been studied deeply for the limited availability of the pure compound. In this paper, a process technology to obtain high purity of DPAn‐3 is introduced and it provides references for the purification of DPAn‐3. In this paper, a process for isolating pure DPAn‐3 ethyl ester from seal oil ethyl esters is proposed. The isolation process comprises four consecutive steps. First, SFAs are removed by urea adduction. Then, molecular distillation is used to remove MUFAs. Next, DPAn‐3 is concentrated from the n‐3PUFAs mixture through AgNO3 complexation. The final purification of DPAn‐3 is accomplished by preparative HPLC. The process proposed in this paper provides an important reference for the purification of DPA. Besides, the process is easy to operate and promising to apply it to industrialization after some large‐scale experiments.