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Immobilization of Candida rugosa Lipase on Glutaraldehyde‐Activated Fe 3 O 4 @Chitosan as a Magnetically Separable Catalyst for Hydrolysis of Castor Oil
Author(s) -
Zhao Ke,
Chen Bang,
Li Cong,
Li XingFu,
Li KeBin,
Shen YeHua
Publication year - 2018
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.201700373
Subject(s) - castor oil , lipase , ricinoleic acid , hydrolysis , nuclear chemistry , glutaraldehyde , chemistry , fourier transform infrared spectroscopy , thermogravimetric analysis , chitosan , materials science , chromatography , organic chemistry , chemical engineering , enzyme , engineering
In this study, the catalyzed hydrolysis of castor oil by Candida rugosa lipase (CRL) immobilized on glutaraldehyde‐activated magnetic Fe 3 O 4 @chitosan is investigated. Magnetic Fe 3 O 4 is prepared by hydrothermal method and coated with chitosan (CS). Next, CRL is immobilized on Fe 3 O 4 @chitosan using glutaraldehyde as a cross‐linking reagent. The prepared Fe 3 O 4 @CS@CRL is confirmed by scanning electron microscopy (SEM), X‐ray powder diffraction (XRD), vibrating sample magnetometry (VSM), Fourier transform infrared (FTIR) spectroscopy, and thermogravimetric analysis (TGA). The response surface methodology (RSM) based on the Box–Behnken design is used to evaluate and optimize the hydrolysis reaction variables. The optimum reaction conditions for the hydrolysis of castor oil by the Fe 3 O 4 @CS@CRL heterogeneous catalyst are found to be a water/oil ratio of 1.60:1, pH of 7.05, reaction temperature of 34 °C, and lipase concentration of 3.27%; under these conditions, the hydrolysis conversion of castor oil reached 46.81%. Moreover, the immobilized lipase showed high stability with no appreciable loss in its activity after three consecutive cycles. Practical Applications: RSM is found to be a useful technique for optimizing hydrolysis of castor oil. The high conversion of the hydrolysis of castor oil indicates that the Fe 3 O 4 @CS@CRL have potential to be used in preparing ricinoleic acid from castor oil. Ricinoleic acid have the potential to be used in printing ink as a pigment and dye disperser, plasticizers, surfactants, lubricants, and other valuable products. Ricinoleic acid can be used in pigment and dye disperser in printing ink, sebacic acid, azelaic acid, heptaldehyde, plasticizers, surfactants, lubricants, and other valuable products. Hydrolysis of castor oil catalyzed by Candida rugosa lipase (CRL) immobilized on glutaraldehyde‐activated magnetic Fe 3 O 4 @chitosan is investigated. The response surface methodology (RSM) based on the Box–Behnken design is used to evaluate and optimize the hydrolysis reaction variables. Fe 3 O 4 @CS@CRL can be easily separated from the suspension using an external strong magnet. Fe 3 O 4 @CS@CRL have potential to be used in preparing ricinoleic acid from castor oil.