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Separation, Purification, and Characterization of Sterol Fatty Acid Esters From Lotus Plumule
Author(s) -
Chen Shuai,
Bi Yanlan,
Chen Jiali,
Wei Maolin,
Sun Shangde,
Chen Jingnan
Publication year - 2017
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.201700139
Subject(s) - chromatography , chemistry , sterol , extraction (chemistry) , elution , acetone , silica gel , fatty acid , adsorption , linoleic acid , column chromatography , oleic acid , organic chemistry , biochemistry , cholesterol
In this work, a new plant resource (lotus plumule) is used to produce sterol fatty acid ester (SFAE). Acetone extraction and column chromatography are developed for the separation and purification of SFAE. Silica gel (200–300 mesh) was the best adsorbent according to the static and dynamic adsorption experiments. The adsorption of SFAE on silica gel (200–300 mesh) is well fitted to Langmuir isotherm model. The column chromatography conditions (elution solvents, loading amount, column height to diameter ratio, and elution flow rate) are optimized and the purity and recovery yield of SFAE are up to 95.56 ± 1.32% and 95.70 ± 3.05%, respectively. Purification processes have no significant effect on sterol composition and fatty acid composition of SFAE. Moreover, SFAE products are identified by UV and IR spectra using standard sample, as well as GC‐MS. GC results show that, β ‐sitosterol (49.6%), △5‐avenasterol (33.4%), and campesterol (9.1%) are the main sterols; and linoleic acid (63.1%), behenic acid (8.3%), and oleic acid (8.1%) are the main fatty acids. Practical Applications : This work provides an economic and efficient method for high purity SFAE production, which gave some available information for the value‐added utilization of idle plant materials with high levels of SFAE. A new plant resource, lotus plumule, is used to produce sterol fatty acid ester (SFAE). Acetone extraction and column chromatography are developed for separation and purification of SFAE. The SFAE product is a pale yellow oily liquid with high purity (95.6%) and recovery (95.7%), which is analyzed and confirmed by GC, MS, and ultraviolet and infrared spectroscopy.