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A fluorescence polarization assay for bisphenol analogs in soybean oil using glucocorticoid receptor
Author(s) -
Guan Tianzhu,
Sun Yonghai,
Yu Hansong,
Li Tiezhu,
Zhang Jie,
Zhang Tiehua
Publication year - 2017
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.201700042
Subject(s) - chromatography , soybean oil , detection limit , analyte , chemistry , glucocorticoid receptor , glucocorticoid , receptor , biochemistry , biology , endocrinology
A fluorescence polarization (FP) assay based on receptor was developed for the determination of bisphenol analogs (BPs) in soybean oil. In the competitive binding system, dexamethasone fluorescein (Dex‐fl) and glucocorticoid receptor (GR) were selected as probe and recognition element, respectively. Crucial factors that affect the precision and accuracy were optimized. After development, the method was characterized by IC 50 values ranging from 1.8 to 66.9 µM of 11 BPs. The established method revealed a limit of detection (LOD) of 1.0 µM and a working range of 7.6–30 µM for BPA in soybean oil. Analysis of spiked soybean oil samples showed that acceptable recoveries ranged from 71.99 to 82.95% with coefficients of variation (CVs) less than 10%. Furthermore, the results detected by the FP assay showed good agreement with that of HPLC with a fit of R 2 = 0.9171. It is concluded that this method offers a reliable alternative for determination of BPs in soybean oil. Practical applications: This work aims to develop FP assay for determination of BPs in soybean oil. Based on the recombinant GR, this method can detect multiple BPs simultaneously. It can potentially be used for high‐throughput screening of BPs, which may serve as an assistant of chromatographic techniques.