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Production of concentrates of CLA obtained from sunflower and safflower and their application to the lipase‐catalyzed acidolysis of egg yolk phosphatidylcholine
Author(s) -
Niezgoda Natalia,
Gliszczyńska Anna,
Gładkowski Witold,
Chojnacka Anna,
Kiełbowicz Grzegorz,
Wawrzeńczyk Czesław
Publication year - 2016
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.201500589
Subject(s) - chemistry , phosphatidylcholine , sunflower oil , lipase , rhizomucor miehei , food science , yolk , chromatography , urea , organic chemistry , triacylglycerol lipase , biochemistry , enzyme , phospholipid , membrane
The process of acidolysis of egg yolk phosphatidylcholine (PC) with concentrates of conjugated linoleic acid (CLA) was performed. CLA was obtained by the alkali isomerization of sunflower and safflower oils. Analysis of the influence of different reaction conditions on the process of isomerization was investigated. Reactions were carried out in preparative scale at 150°C in the ethylene glycol and afforded the mixture of isomers: cis ‐9, trans ‐11 and trans ‐10, cis ‐12. Their content was 30.8 and 32.3% for product obtained from sunflower oil and 30.4 and 36.4% for the product obtained from safflower oil. Crude concentrates were purified by urea complexation with 1:3 fatty acids to urea weight ratio. Enzymatic modification of phosphatidylcholine from egg yolk with CLA concentrates was carried out using lipase from Rhizomucor miehei . The process was performed at 45°C with controlled water activity. The highest effective incorporation (42%) of CLA into PC and lysophosphatidylcholine molecules was obtained using concentrate from the sunflower and safflower oils after 36 h of reaction. Practical applications: Results of this research are useful for the development of scaled up acidolysis reaction to produce phosphatidylcholine enriched in biologically active isomers of CLA using the sunflower or safflower oil as a rich source of linolenic acid. Application of the concentrates of CLA obtained from sunflower and safflower oils to the enzymatic acidolysis of egg yolk phosphatidylcholine.