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Substrate specificity of Mortierella alpina Δ9‐III fatty acid desaturase and its value for the production of omega‐9 MUFA
Author(s) -
Hao Guangfei,
Chen Haiqin,
Yang Bo,
Du Kai,
Wang Hongchao,
Gu Zhennan,
Zhang Hao,
Chen Wei,
Chen Yong Q.
Publication year - 2016
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.201500257
Subject(s) - palmitic acid , stearic acid , palmitoleic acid , oleic acid , fatty acid , chemistry , biochemistry , substrate (aquarium) , strain (injury) , saturated fatty acid , food science , biology , organic chemistry , ecology , anatomy
The fatty acid desaturase (FADS9‐III) gene from M. alpina ATCC 32222 was cloned and overexpressed in a homologous system. Lipid analysis via GC‐MS revealed that the Δ9‐III fatty acid desaturase had moderate activity with palmitic acid (C16:0) and stearic acid (C18:0) as substrates. Palmitoleic acid (C16:1) and oleic acid (C18:1) contents increased 4.0‐ and 1.94‐fold in the FADS9‐III overexpression clones compared to the wild‐type strain. Low temperature (12°C) significantly improved the conversion ratio of C16:0 to C16:1 (9.12‐fold increase). In addition, the FADS9‐III fatty acid desaturase had specific activity at the ω9 position with lignoceric acid (C24:0) as a substrate. Practical applications: Our results indicate that FADS9‐III prefers stearic acid (C18:0) and palmitic acid (C16:0) as substrates and has ω9 fatty acid desaturase activity on lignoceric acid (C24:0). The conversion ratio of C24:0 to C24:1 reached 17% by supplementation of C24:0 in the medium at low temperature. The M. alpina ω9 fatty acid desaturase (FADS9‐III) is valuable for the production of mono‐unsaturated fatty acids with a double bond at the ω9 position. The M. alpina Δ9‐III fatty acid desaturase is valuable for production of MUFA with the double bond at the ω9 position.