Premium
Beyond diazomethane: Alternative approaches to analyzing non‐esterified fatty acids
Author(s) -
Potter Greg,
Budge Suzanne M.,
Speers R. Alex
Publication year - 2015
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.201400404
Subject(s) - diazomethane , nefa , derivatization , chemistry , reagent , chromatography , extraction (chemistry) , organic chemistry , methylation , fatty acid , biochemistry , high performance liquid chromatography , gene
In many branches of lipid science, researchers are interested in non‐esterified fatty acid (NEFA) content and composition. For many years, diazomethane was the reagent of choice to selectively derivatize and then detect NEFA due to its highly specific methylation of the carboxylic acid functional group. While the activity of this derivatizing reagent is very defined, it is dangerous and can be difficult to obtain. In this brief review, we have compiled a collection of methods which allow for the detection of NEFA and hydroxy NEFA without the use of diazomethane. We have concentrated on methods that employ three distinct approaches of selective quantification/extraction, purification from total lipids and derivatization techniques. Chemical reactions that occur during selective extraction of NEFA using a quaternary ammonium salt (a) and the later pyrolytic derivatization when the mixture is placed in the hot injection port of a GC (b).