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Metal ions can affect the composition and production of sophorolipids by Wickerhamiella domercqiae Y 2A CGMCC 3798
Author(s) -
Chen Jing,
Zhang Hongrui,
Liu Yue,
Fu Shengmi,
Liu Xinli
Publication year - 2014
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.201300512
Subject(s) - metal ions in aqueous solution , fermentation , chemistry , composition (language) , metal , ion , nuclear chemistry , chromatography , food science , materials science , organic chemistry , art , literature
Different metal ions were added as supplements to culture media to study sophorolipids production by Wickerhamiella domercqiae Y 2A CGMCC 3798. The fermentation media were supplemented with different metal ions in the form of sulfate. A maximum yield 149.90 g/L was achieved in the presence of Cu 2+ ions. The production of lactonic sophorolipids was favored when culture media wad supplied with Mg 2+ ions, while Fe 2+ ions were the optimal ions for acidic sophorolipids production. TLC, HPLC, and LC–MS analyses were used for further investigation on the sophorolipids production. The results of SDS–PAGE and enzymatic transformation of acidic sophorolipids showed differential expression of extracellular proteins of W. domercqiae Y 2A CGMCC 3798 in presence of Mg 2+ or Fe 2+ . Overall the results indicated potential selective production of different sophorolipids based on selected metal ions in culture media. Practical applications: During the fermentation process of sophorolipids, L‐SLs and A‐SLs were produced selectively by adding Mg 2+ and Fe 2+ into fermentation media, respectively. Sophorolipid composition and production are different produced by Wickerhamiella domercqiae Y 2A CGMCC 3798 cultured with Mg 2+ (a) and Fe 2+ (b), respectively by HPLC analysis.

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