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Toxicity testing of sildenafil base‐loaded liposomes in in vitro and ex vivo models for pulmonary application
Author(s) -
Paranjpe Mukta,
Neuhaus Vanessa,
Braun Armin,
MuellerGoymann Christel C.
Publication year - 2014
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.201300406
Subject(s) - ex vivo , liposome , in vivo , pharmacology , toxicity , chemistry , a549 cell , in vitro , cytotoxicity , medicine , biochemistry , biology , microbiology and biotechnology , organic chemistry
The aim of this study was to formulate sildenafil base‐loaded liposomes using a solvent‐free method, followed by the investigation of the potential cytotoxicity of the prepared liposomes in in vitro and ex vivo cell culture models. The liposomes were manufactured by dispersing 5% w/v phospholipid in aqueous phase at RT. The prepared dispersion was then homogenized using an Avestin extruder. The particle size of the prepared liposomes was between 100 and 110 nm and was stable up to 6 months. For in vitro models, human alveolar epithelial cell line (A549) and mouse heart endothelium cell line (MHEC5‐T) were used. For ex vivo models, rat precision cut lung slices (PCLS) and rat heart slices (PCHS) were used. All the models were treated with liposomes loaded with or without sildenafil in a concentration range of 0–2500 µg/mL of phospholipid. The toxicity was assessed in vitro and ex vivo using MTT assay. Median lethal dose (LD 50 ) values for A549 cells and PCLS were found between 800 and 1800 µg/mL while for MHEC5‐T cells and PCHS they were found between 1000 and 1200 µg/mL. PCHS demonstrated higher LD 50 values as compared to PCLS. Considering the high LD 50 values, sildenafil‐loaded liposomes may have the potential for the treatment of pulmonary hypertension via inhalation route. Practical applications: The liposomes have a potential for administration in lungs. Toxicity study was performed to determine a safe dose for potential human administration. The use of solvents for the preparation of liposomes using the conventional dried lipid film method can be avoided.