Premium
Separation and identification of lipid classes by normal phase LC‐ESI/MS/MS on a cyanopropyl column
Author(s) -
Olsson Petter,
Holmbäck Jan,
Nilsson Ulrika,
Herslöf Bengt
Publication year - 2014
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.201300291
Subject(s) - chemistry , chromatography , electrospray ionization , electrospray , mass spectrometry , tandem mass spectrometry , hexane , high performance liquid chromatography , lipidomics , selected reaction monitoring , adduct , liquid chromatography–mass spectrometry , analytical chemistry (journal) , organic chemistry , biochemistry
In order to establish a versatile and convenient method for the analysis of lipids, electrospray ionization tandem mass spectrometry (ESI‐MS/MS) was applied to a HPLC separation on a cyanopropyl‐bonded stationary phase. A binary gradient mobile phase system consisting of hexane, toluene, methanol and a stable electrospray yielding sodium adduct ions could be used to generate specific product ions in MS/MS mode. By applying the LC/ESI‐MS/MS method on an egg yolk sample, 29 different molecular species of phosphatidylethanolamines, phosphatidylcholines, and lysophosphatidylcholines could be detected within 25 min.