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Enrichment process for α‐linolenic acid from silkworm pupae oil
Author(s) -
Wang Jun,
Zhang JinLiang,
Wu FuAn
Publication year - 2013
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.201200324
Subject(s) - polyunsaturated fatty acid , chemistry , linoleic acid , linolenic acid , oleic acid , urea , food science , fatty acid , ethanol , unsaturated fatty acid , biochemistry , chromatography
The desilked silkworm ( Bombyx mori L.) pupae oil is a good source of α‐linolenic acid (ALA), but selectively enriching ALA is a great technical challenge due to the presence of multiple analogues such as linoleic acid and oleic acid. To set up a simple, highly selective and low‐cost combinative inclusion process for obtaining ALA concentrate from silkworm pupae oil, the effects of β‐cyclodextrin (β‐CD) inclusion parameters on the purity and recovery of polyunsaturated fatty acid (PUFA) were investigated; then, four types of combinative processes using β‐CD and/or urea were compared. The results indicated that a two‐stage combinative inclusion process was established. In the first stage, when the volume ratio of β‐CD‐saturated solution to free fatty acids ethanol solution was 9 v/v, the dispersion temperature and time were 60°C and 1.5 h, the freezing temperature and time were −10°C and 15 h, and the purity of ALA was raised from 15.0 to 47.6% with a recovery of 39.4%. Then, the purity of ALA was further increased to 67.4% in the second‐stage operation with a corresponding recovery of 34.8% using urea complexation.