Premium
Synthesis of CLA‐enriched TAG by Candida antarctica lipase under vacuum
Author(s) -
Hong Seung In,
Kim Yangha,
Yoon Sung Won,
Cho Seung Yong,
Kim InHwan
Publication year - 2012
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.201100233
Subject(s) - candida antarctica , conjugated linoleic acid , lipase , glycerol , chemistry , enzyme , linoleic acid , chromatography , biochemistry , fatty acid
Conjugated linoleic acid (CLA)‐enriched triacylglycerol (TAG) of 90 wt% was successfully synthesized in 10 h by direct esterification of glycerol and CLA using an immobilized lipase from Candida antarctica under vacuum. The best operating conditions for the synthesis of TAG were investigated according to the three parameters of temperature, enzyme loading, and vacuum. The synthesis of TAG increased with increasing temperature but it did not significantly change above 60°C ( p >0.05). The increase of enzyme loading lead to an enhanced conversion of TAG, but enzyme loading of more than 10% (based on the total weight of the substrates) was not effective. Moreover, when vacuum increased, the conversion of TAG increased, but the conversion rate decreased when the vacuum level was too high. The best combination of temperature, enzyme loading, and vacuum level were 60°C, 10% of the total weight of the substrates, and 0.4 kPa, respectively. During the initial 6 h of reaction, Candida antarctica lipase had more selectivity for 10 t ,12 c ‐CLA than 9 c ,11 t ‐CLA onto the glycerol backbone, and a preference for the incorporation at the sn ‐1,3 positions of glycerol rather than at the sn ‐2 position.