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Effects of dietary n−3/n−6 ratio on lipid metabolism of gilthead seabream ( Sparus aurata )
Author(s) -
Bandarra Narcisa M.,
Rema Paulo,
Batista Irineu,
PousãoFerreira Pedro,
Valente Luisa M. P.,
Batista Sónia M. G.,
Ozório Rodrigo O. A.
Publication year - 2011
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.201100087
Subject(s) - docosapentaenoic acid , eicosapentaenoic acid , polyunsaturated fatty acid , chemistry , feed conversion ratio , food science , metabolism , zoology , protein efficiency ratio , linoleic acid , fatty acid , biology , biochemistry , endocrinology , body weight
The current study was conducted to evaluate the effects of various dietary n−3/n−6 ratio on growth and lipid metabolism in gilthead seabream ( Sparus aurata ) juveniles. The fish were fed ad libitum on three isoproteic (52%), isolipidic (14%), and isoenergetic diets (21 MJ/kg), formulated to contain three different n−3/n−6 ratios: a high (3.8), medium (2.5), or low (0.9) ratio. Fish grew from 9.5 ± 0.1 g to 54.2 ± 0.7 g in 12 weeks. Growth rate (2.2% BW/day), voluntary feed intake (2.4 ± 0.1% BM/day), feed conversion ratio (1.38 ± 0.05), and net protein utilization (27.5 ± 1.0%) did not vary among treatments. In contrast, body lipid content and lipid deposition efficiency were significantly reduced in fish fed low n−3/n−6 ratio. Fish fed the highest n−3/n−6 ratio showed the lowest hepatic glucose‐6‐phosphate dehydrogenase (G6PD) activity ( p = 0.02). The n−3/n−6 ratio decreased, while DHA/eicosapentaenoic acid (EPA) ratio increased significantly with decreasing dietary n−3/n−6 content. Among n−3 PUFA, the DHA and, particularly, the docosapentaenoic acid (DPA) showed the highest deposition rate both in muscle and liver. The lower deposition rate (<1) of linoleic acid (LA) and linolenic acid (LNA) suggests that a reduction in dietary n−3/n−6 ratio may stimulate their transport to the intermediary metabolism for energy production.