Premium
Effects of Orlistat on white adipose tissue (WAT) in the γ‐irradiated mouse model
Author(s) -
Roh Changhyun,
Choi NamHee,
Park HaeRan,
Jung Uhee,
Jo Sung Kee
Publication year - 2010
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.201000423
Subject(s) - orlistat , adipose tissue , white adipose tissue , endocrinology , medicine , triglyceride , chemistry , weight loss , obesity , irradiation , cholesterol , biology , physics , nuclear physics
In this study, we showed that γ‐irradiation could trigger biological response like the fat accumulation of gonadal white adipose tissue (gWAT) in mice. The irradiated animal can be used as a useful model for evaluation of anti‐obesity drugs. To induce the fat accumulation by γ‐irradiation, 2‐months‐old female C57BL/6 mice were irradiated at 5 Gy and further raised for 6 months. Subsequently, the mice were i.p. injected daily with Orlistat (25 mg/kg) or vehicle for 3 wk and analyzed for the adipose tissue weight and serum triglyceride and T‐cholesterol levels. The abdominal WAT of the γ‐irradiated mice weighed an average of 3.9/100 g body weight, 1.7‐fold higher than what was seen in normal mice (2.3/100 g body weight), indicating that γ‐irradiation induced the fat accumulation in the adipose tissue. However, the administration of Orlistat, a well‐known anti‐obesity agent, significantly reduced the adipose tissue weight to 1.7/100 g body weight in irradiated mice. In addition, in these Orlistat‐treated mice, a significant reduction of serum triglyceride and T‐cholesterol was observed up to 14% and 27%, respectively.