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Degumming rice bran oil using phospholipase‐A 1
Author(s) -
Manjula Sarode,
Jose Anju,
Divakar Soundar,
Subramanian Rangaswamy
Publication year - 2011
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.201000376
Subject(s) - chemistry , citric acid , rice bran oil , phospholipase a1 , solvent , aqueous two phase system , food science , bran , phosphorus , chromatography , aqueous solution , pulp and paper industry , phospholipase , enzyme , biochemistry , organic chemistry , raw material , engineering
The efficacy of enzymatic degumming was assessed using the third generation phospholipase‐A 1 , Lecitase®‐Ultra (EC 3.1.1.3) from Thermomyces lanuginosa / Fusarium oxysporum with different qualities of crude rice bran oil. The phosphorus content in the oil reduced to ∼10 mg/kg from an initial level of 390 mg/kg after 2 h of incubation period at 50°C. However, in the solvent‐phase degumming, there was practically no phospholipid reduction at lower water content (2%) due to the poor contact between the highly nonpolar solvent and the aqueous phase (citric acid, NaOH, and enzyme solutions). Increasing the water content to 20% reduced the phosphorus level in the degummed‐oil to 71 mg/kg but did not match the performance of oil‐phase degumming. The degumming efficiency of Lecitase®‐Ultra was effective in oil‐phase and suitable for practical application. Solvent‐phase enzymatic degumming offers more benefits but needs greater efforts to overcome the challenges.