Premium
Anionic long‐circulating liposomes for delivery of radioiodinated antisense oligonucleotides
Author(s) -
Chao Ma,
Jiawei Xie,
Zhongxin Jiang,
Kuang Anren
Publication year - 2010
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.200900283
Subject(s) - liposome , chemistry , oligonucleotide , antisense therapy , drug delivery , zeta potential , dispersity , pharmacology , drug carrier , toxicity , microbiology and biotechnology , biochemistry , medicine , gene , nanotechnology , biology , materials science , nanoparticle , organic chemistry , locked nucleic acid
Long‐circulating liposomes have been widely used to enhance efficacy of gene therapy. Antisense therapy might increase the efficacy of radiation or chemotherapy; therefore, we undertook to optimize the composition of the liposomal delivery vehicles. The radiolabeling efficiency, radiochemistry purity, and specific radioactivity of radioiodinated antisense oligonucleotides (ASON) were 71.66 ± 7.73, 98.33 ± 0.39%, 4.09 ± 0.11 MBq/nmol. Radioiodinated ASON remained stable in 0.01 M HEPES buffer and human serum even after incubation for 4 hours. Mean diameter of the anionic long‐circulating liposomes (ALCL) was 504 ± 31.76 nm with a polydispersity index (PDI) of 0.107 ± 0.008 before extrusion, 115 ± 8.5 nm with a PDI of 0.103 ± 0.002 after extrusion. The zeta potential of ALCL was −29.23 ± 0.45 mV. ALCL prepared for this study provided 70.28 ± 1.84% encapsulation efficiency. Compared with other liposome formulations, the ALCL mediated enhanced cellular uptake (32.51 ± 1.44%) by MCF‐7 breast cancer cells ( p <0.05). Practical applications: Drug delivery systems can in principle provide enhanced efficacy and/or reduced toxicity for anticancer agents. LCL have become a commonly used carrier for gene therapy recently. ALCL are promising for the mediated radiation and antisense therapy for breast cancer which is the leading cause for women.