Premium
Improvement of mono and diacylglycerol production via enzymatic glycerolysis in tert ‐butanol system
Author(s) -
Krüger Roberta L.,
Valério Alexsandra,
Balen Manuela,
Ninow Jorge Luiz,
Vladimir Oliveira J.,
de Oliveira Débora,
Corazza Marcos Lúcio
Publication year - 2010
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.200900253
Subject(s) - chemistry , glycerol , lipase , butanol , chromatography , diacylglycerol kinase , molar ratio , solvent , n butanol , catalysis , enzyme , organic chemistry , ethanol , protein kinase c
In this work we report experimental data regarding the glycerolysis of olive oil using Novozym 435 in tert ‐butanol organic system aiming at the production of monoacylglycerols (MAG) and diacylglycerols (DAG). Experiments were performed in batch mode, recording the reaction kinetics and evaluating the effects of temperature, enzyme concentration, tert ‐butanol:oil/glycerol volume ratio and using solvent to substrates ratio of 1:1 and 5:1 v/v. Experimental results showed that lipase‐catalyzed glycerolysis in tert ‐butanol might be a potential route for the production of high contents of MAG and DAG. The results also showed that it is possible to maximize the production of MAG and/or DAG, depending on the glycerol to oil molar ratio employed in the reactional system. Higher contents of MAG (53 wt%) and DAG (50 wt%) were achieved using glycerol to oil molar ratio of 3:1/6:1 and 0.5:1.5, respectively, both in 8 h of reaction at 70°C, 600 rpm and enzyme concentration of 10 wt%.